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Lab. Plant Molecular Genetics - Nakagawa & Hachiya Lab. HOME > Gateway Binary Vector (pGWB)


Information of Gateway Binary Vector (pGWB)
Gateway Binary Vector with organelle-targeted fluorescent protein >> Gateway Vector for BiFC >> Gateway Recycling Cloning >> Dual-Site Gateway Cloning >>

Review (INTECH Open Access): Gateway Vectors for Plant Genetic Engineering: Overview of Plant Vectors, Application for Bimolecular Fluorescence Complementation (BiFC) and Multigene Construction


attR1-attR2 type pGWB series (pBI backbone, Kmr and Hygr for bacteria and plant)

Complete List of pGWB　PDF download pGWB1-45 (pBI backbone, PNOS:NTPII and P35S:HPT) (List) pGWB203-235 (pBI backbone, PNOS:NTPII and PNOS:HPT) (List) manual Japanese (Word / PDF) manuals　　Word / PDF manual English (Word / PDF) manuals　　Word / PDF Reference Most Cited Journal of JBB (last five years) pGWB1-45 and 203-235 are planed to be deposited at RIKEN in future. We will announce when the deposit is completed.
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attR1-attR2 type ImpGWB series (pPZP backbone, SpcR for bacteria, KmR or HygR or BASTA-R or TunicamycinR for plant)

Complete List of ImpGWB　PDF download pGWB401-461 (KmR, pPZP backbone, PNOS:NTPII) (List) Reference pGWB401-455 are available from Addgene. pGWB401-461 are available from RIKEN BRC Experimental Plant Division. pGWB501-561 (HygR, pPZP backbone, PNOS:HPT) (List) Reference pGWB501-555 are available from Addgene. pGWB501-555 are available from RIKEN BRC Experimental Plant Division. pGWB601-661 (BASTA-R, pPZP backbone, PNOS:bar) (List) Reference pGWB601-661 are available from RIKEN BRC Experimental Plant Division. pGWB701-761 (TunicamycinR, pPZP backbone, PNOS:GPT) (List) Reference Comments on tunicamycin selection (important) manual Japanese (PDF) manual English (PDF) pGWB with organelle-targeted fluorescent protein pGWB with organelle-targeted fluorescent protein RIKEN BRC Experimental Plant Division
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attR4-attR2 type (Promoter Swapping) R4pGWB series (pPZP backbone, SpcR for bacteria, KmR or HygR or BASTA-R or TunicamycinR for plant)

Complete List of R4pGWB　PDF download R4pGWB401-459 (KmR, pPZP backbone, PNOS:NTPII) (List) Reference pGWB401-459 are available from RIKEN BRC Experimental Plant Division. R4pGWB501-559 (HygR, pPZP backbone, PNOS:HPT) (List) Reference pGWB501-559 are available from RIKEN BRC Experimental Plant Division. R4pGWB601-659 (BASTA-R, pPZP backbone, PNOS:bar) (List) Reference pGWB601-659 are available from RIKEN BRC Experimental Plant Division. R4pGWB701-759 (TunicamycinR, pPZP backbone, PNOS:GPT) (List) Reference Comments on tunicamycin selection (important) manual Japanese (PDF) manual English (PDF) ● Information concerning pDONR P4-P1R (Donor vector for construction of attL4-promoter-attR1 entry clones) The sale of pDONR P4-P1R (MultiSite Gateway Three-Fragment Vector Construction Kit) was terminated. Instead of pDONR P4-P1R, you may use pENTR™ 5'-TOPO™ TA Cloning™ Kit for construction of attL4-promoter-attR1 entry clones. https://www.thermofisher.com/order/catalog/product/K59120?ICID=search-k59120
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attR4-attL1type (Promoter Analysis) R4L1pGWB series (pPZP backbone, SpcR for bacteria, KmR or HygR or BASTA-R for plant)

Complete List of R4L1pGWB　PDF download R4L1pGWB432-459 (KmR, pPZP backbone, PNOS:NTPII) (List) Reference R4L1pGWB532-559 (HygR, pPZP backbone, PNOS:HPT) (List) Reference R4L1pGWB632-659 (BASTA-R, pPZP backbone, PNOS:bar) (List) Reference MTA for BASTA-R R4L1 vectors Japanese (Word / PDF) MTA for BASTA-R R4L1 vectors English (Word / PDF) R4L1pGWB732-759 (TunicamysinR, pPZP backbone, PNOS:GPT (List) Reference Comments on tunicamycin selection (important) manual Japanese (PDF) manual English (PDF) pGWB with organelle-targeted fluorescent protein pGWB with organelle-targeted fluorescent protein RIKEN BRC Experimental Plant Division ● Information concerning pDONR P4-P1R (Donor vector for construction of attL4-promoter-attR1 entry clones) The sale of pDONR P4-P1R (MultiSite Gateway Three-Fragment Vector Construction Kit) was terminated. Instead of pDONR P4-P1R, you may use pENTR™ 5'-TOPO™ TA Cloning™ Kit for construction of attL4-promoter-attR1 entry clones. https://www.thermofisher.com/order/catalog/product/K59120?ICID=search-k59120
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Gateway Binary Vector with organelle-targeted fluorescent protein pGWB series (attR1-attR2 type, pPZP ackbone, SpcR for bacteria, KmR or HygR or BASTA-R or TunicamycinR for plant) R4L1pGWB series (attR4-attL1 type, pPZP backbone, SpcR for bacteria, KmR or HygR or BASTA-R or TunicamycinR for plant)

Complete List of pGWB and R4L1pGWB with organelle-targeted fluorescent protein　PDF download pGWB462-491, R4L1pGWB462-491 (KmR, pPZP backbone, PNOS:NTPII) (List) Reference pGWB562-591, R4L1pGWB562-591 (HygR, pPZP backbone, PNOS:HPT) (List) Reference pGWB662-691, R4L1pGWB662-691 (BASTA-R, pPZP backbone, PNOS:bar) (List) Reference pGWB762-791, R4L1pGWB762-791 (TunicamycinR, pPZP backbone, PNOS:GPT) (List) Reference pGWB with organelle-targeted fluorescent protein RIKEN BRC Experimental Plant Division Comments on tunicamycin selection (important) ● Information concerning pDONR P4-P1R (Donor vector for construction of attL4-promoter-attR1 entry clones) The sale of pDONR P4-P1R (MultiSite Gateway Three-Fragment Vector Construction Kit) was terminated. Instead of pDONR P4-P1R, you may use pENTR™ 5'-TOPO™ TA Cloning™ Kit for construction of attL4-promoter-attR1 entry clones. https://www.thermofisher.com/order/catalog/product/K59120?ICID=search-k59120
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Gateway Vector for BiFC (not binary) Gateway Vector for BiFC (pUC119 backbone, Ampr for bacteria)

Complete List of Gateway Vector for BiFC　PDF download pGWnY (nYFP/pUGW2) (List) pnYGW (nYFP/pUGW0) pGWcY (cYFP/pUGW2) pcYGW (cYFP/pUGW0) Reference Reference for former name
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Gateway Recycling Cloning Ver. 1 Gateway Vector for Recycling Cloning Ver. 1 (pDONR201 backbone, Km^r for bacteria)

Complete List of Gateway Vector for Recycling Cloning Ver. 1　PDF download pRED419 (List) pRED418 pCON Reference NOTE Multiple use of CaMV35S promoters frequently causes silencing in this system.
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Gateway Recycling Cloning Ver. 2 Gateway Vector for Recycling Cloning Ver. 2 (pDONR201 backbone, Km^r for bacteria)

Improvements in Ver.2 (1) Single digestion at RCS prior to LR reaction is enough for Ver.2 (Double digestion at RCS is necessary for Ver. 1). (2) Ampicillin selection is available in transformation after LR reaction using pRED419-Amp or pRED418-Amp. (3) Chloramphenicol selection is available in transformation after LR reaction using pCON-Cm-rare1 or pCON-Cm-rare2. (4) Use of ampicillin (chloramphenicol) greatly enhances selection of correctly constructed clones in transformation. Complete List of Gateway Vector for Recycling Cloning Ver. 2　PDF download pRED419-Amp (List) pRED418-Amp pCON-Cm-rare1 pCON-Cm-rare2 Reference In preparation NOTE Multiple use of CaMV35S promoters frequently causes silencing in this system.
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R4 Dual-Site (R4DS) Gateway Cloning System Cloning of two set of promoter and ORF into a binary vector. R4pGWB6xxx-MD8 series (pPZP backbone. SpcR for bacteria. KmR or HygR or BASTA-R or TunicamycinR for plant) [Intermediate vector R4pDD6xx-MD8 series (pUC119 backbone. AmpR for bacteria)]

Complete List of R4DS Gateway Cloning System　PDF download R4pGWB6401-MD8 to R4pGWB6459-MD8, R4pGWB6400-MD8-n/cYFP (KmR, pPZP backbone, Pos:NPTII) (List) Reference R4pGWB6501-MD8 to R4pGWB6559-MD8, R4pGWB6500-MD8-n/cYFP (HygR, pPZP backbone, Pos:HPT) (List) Reference R4pGWB6601-MD8 to R4pGWB6659-MD8, R4pGWB6600-MD8-n/cYFP (BASTA-R, pPZP backbone, Pos:bar) (List) Reference R4pGWB6701-MD8 to R4pGWB6759-MD8, R4pGWB6700-MD8-n/cYFP (TunicamycinR, pPZP backbone, Pnos:GPT) (List) Reference R4pDD601-MD8 to R4pDD659-MD8, R4pDD600-MD8-n/cYFP (AmpR, pUC119 backbone) (List) Reference All vectors of R4DS Gateway Cloning System are available from RIKEN BRC Experimental Plant Division. ● Information concerning pDONR P4-P1R (Donor vector for construction of attL4-promoter-attR1 entry clones) The sale of pDONR P4-P1R (MultiSite Gateway Three-Fragment Vector Construction Kit) was terminated. Instead of pDONR P4-P1R, you may use pENTR™ 5'-TOPO™ TA Cloning™ Kit for construction of attL4-promoter-attR1 entry clones. https://www.thermofisher.com/order/catalog/product/K59120?ICID=search-k59120
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Dual-Site (DS) Gateway Cloning System (Pnos) Cloning of two ORF into binary vector. Expression by Pnos (nopaline synthase promoter). N-terminal fusion with 14 tags are available. N-tarminal and C-terminal fusion with n/cYFP are available. pGWB6xxx-MD8-Pnos series (pPZP backbone, SpcR for bacteria, KmR or HygR or BASTA-R or Tunicamycin R for plant) [Intermediate vector pDD6xx-MD8-Pnos series (pUC119 backbone. AmpR for bacteria)]

Complete List of DS Gateway Cloning System (Pnos) PDF download pGWB6402-MD8-Pnos to pGWB6461-MD8-Pnos, pGWB6400-MD8-Pnos-N/CY0/2 (KmR, pPZP backbone, Pnos:NPTII) (List) Reference pGWB6502-MD8-Pnos to pGWB6561-MD8-Pnos, pGWB6500-MD8-Pnos-N/CY0/2 (HygR, pPZP backbone, Pnos:HPT) (List) Reference pGWB6602-MD8-Pnos to pGWB6661-MD8-Pnos, pGWB6600-MD8-Pnos-N/CY0/2 (BASTA-R, pPZP backbone, Pnos:bar) (List) Reference pGWB6702-MD8-Pnos to pGWB6761-MD8-Pnos, pGWB6700-MD8-Pnos-N/CY0/2 (TunicamycinR, pPZP backbone, Pnos:GPT) (List) Reference pDD602-MD8-Pnos to pDD661-MD8-Pnos, pDD600-MD8-Pnos-N/CY0/2 (AmpR, pUC119 backbone) (List) Reference All vectors of DS Gateway Cloning System (Pnos) are available from RIKEN BRC Experimental Plant Division.
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pENTR-NeCo-lacZa System For preparation of empty vector (negative control construct) in Gateway cloning

Complete List of pENTR-NeCo-lacZa PDF download Reference All pENTR-NeCo-lacZa vectors are available from RIKEN BRC Experimental Plant Division.